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Curr Pharm Des This article has been cited by other articles in PMC. Transforming growth factor beta activation of c-Abl is independent of receptor internalization and regulated by phosphatidylinositol 3-kinase and PAK2 in mesenchymal cultures. Mechanism of activation of the TGF-beta receptor. Next, the same double IF technique was performed on liver biopsies of patients with colorectal cancer that were obtained from a Mayo Clinic tissue collection

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These conditioned media were used as stimulants in cell proliferation assays and chemoattractants in Boyden chamber assays. Data represent 3 repeats with similar results.

Post-translational regulation of TGF-beta receptor and Smad signaling. Further analysis and quantification were done as previously described 5. Furthermore, our study is also highly relevant to other diseases that are associated with myofibroblastic activation, including liver, lung, and renal fibrosis.

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Differential properties of current tyrosine kinase inhibitors in gastrointestinal stromal tumors. Cytokine Growth Factor Rev Mechanisms of TGF-beta signaling from cell membrane to the nucleus. No conflicts of interest, financial or otherwise, are declared by the authors.

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Immunoprecipitation and Western blot. WB was carried out as we previously described 5.

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J Biol Chem Immunofluorescence, confocal microscopy, and image quantification. Retroviral and lentiviral transduction of HSCs. STEM is an extensively used antibody for detection of human-derived cells in mice, owing to its specific reaction with a human cytoplasmic antigen. As shown in Fig. Induction of c-sis mRNA and activity similar to platelet-derived growth factor by transforming growth factor beta: Mol Biol Cell Mechanisms of hepatic fibrogenesis.

Data represent 3 independent experiments with consistent results. Role of platelet-derived growth factors in physiology and medicine. Cells were harvested for Western blot WB.

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Received Apr 9; Accepted Aug Liver biopsies of patients with colorectal cancer were from a Mayo Clinic tissue collection, and the clinical features of the patients were described in a recent publication National Center for Biotechnology InformationU. Densitometric analysis is shown on the bottom.

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Arterioscler Thromb Vasc Biol Shah, and a startup fund to N. Transforming growth factor beta signaling via Ras in mesenchymal cells requires pactivated kinase 2 for extracellular signal-regulated kinase-dependent transcriptional responses.

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Nitric oxide regulates tumor cell cross-talk with stromal cells in the tumor microenvironment of the liver. Quiescent HSCs were negative for both proteins arrowheads.

Author information Article notes Copyright and License information Disclaimer. Selective upregulation of platelet-derived growth factor alpha receptors by transforming growth factor beta in scleroderma fibroblasts. Am J Pathol GAPDH was used as an internal control.